- Animal Park at the Conservators Center
- Asheville Museum of Science
- Aurora Fossil Museum
- Bald Head Island Conservancy
- Cape Fear Botanical Garden
- Cape Fear Museum of History & Science
- Carolina Beach State Park
- Carolina Raptor Center
- Carvers Creek State Park
- Catawba Science Center
- Charlotte Museum of Nature
- Chimney Rock State Park
- Cliffs of Neuse State Park
- Contentnea Creek
- Core Sound Waterfowl Museum & Heritage Center Willow Pond Trail
- Cowan Museum of History and Science
- Cradle of Forestry
- Crowders Mountain State Park
- Daniel Stowe Botanical Garden
- Discovery Place Kids-Huntersville
- Discovery Place Kids-Rockingham
- Discovery Place Science
- Dismal Swamp State Park
- Elk Knob State Park
- Eno River State Park
- Falls Lake State Recreation Area
- Fascinate-U Children's Museum
- Fort Fisher State Recreation Area
- Fort Macon State Park
- Goose Creek State Park
- Gorges State Park
- Grandfather Mountain State Park
- Grandfather Mountain Stewardship Foundation
- Greensboro Science Center
- Hammocks Beach State Park
- Hands On! Children's Museum
- Hanging Rock State Park
- Haw River State Park
- Highlands Biological Station / Nature Center
- Imagination Station Science and History Museum
- Imperial Centre for the Arts & Sciences
- Iredell Museums
- Jennette's Pier
- Jockey's Ridge State Park
- Jones Lake State Park
- Jordan Lake State Recreation Area
- Kaleideum
- Kerr Lake State Recreation Area
- KidSenses Children's Interactive Museum
- Lake James State Park
- Lake Norman State Park
- Lake Waccamaw State Park
- Lumber River State Park
- Marbles Kids Museum
- Mayland Earth to Sky Park
- Mayo River State Park
- Medoc Mountain State Park
- Merchants Millpond State Park
- Miriam P. Brenner Children's Museum
- Morehead Planetarium & Science Center
- Morrow Mountain State Park
- Mount Jefferson State Natural Area
- Mount Mitchell State Park
- Muddy Sneakers
- Museum of Coastal Carolina and Ingram Planetarium
- Museum of Life and Science
- NC Aquarium at Pine Knoll Shores
- NC Museum of Natural Sciences at Whiteville
- New River State Park
- North Carolina Aquarium at Fort Fisher
- North Carolina Aquarium on Roanoke Island
- North Carolina Arboretum
- North Carolina Botanical Garden
- North Carolina Estuarium
- North Carolina Museum of Natural Sciences
- North Carolina Museum of Natural Sciences at Greenville
- North Carolina Transportation Museum
- North Carolina Zoo
- Pettigrew State Park
- Pilot Mountain State Park
- Pisgah Astronomical Research Institute (PARI)
- Port Discover
- Prairie Ridge Ecostation
- Raven Rock State Park
- Rendezvous Mountain State Park
- Roanoke/Cashie River Center
- Robeson Planetarium and Science Center
- Rock Museum
- Rowan Wild and Dan Nicholas Park
- Schiele Museum
- Seeds of Hope Wilson
- Singletary Lake State Park
- South Mountains State Park
- Stone Mountain State Park
- Sturgeon City Environmental Education Center
- Sullenberger Aviation Museum
- Sylvan Heights Bird Park
- Team ECCO / Aquarium & Shark Lab by Team ECCO
- The Children's Museum of Wilmington
- The Nest
- Vollis Simpson Whirligig Park and Museum
- Weymouth Woods Sandhills Nature Preserve
- White Deer Park Nature Center
- William B. Umstead State Park
- WNC Nature Center